6CVT
Human Aprataxin (Aptx) V263G bound to RNA-DNA, AMP and Zn product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.727, 113.131, 124.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.961 - 2.941 |
| R-factor | 0.2482 |
| Rwork | 0.246 |
| R-free | 0.28920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ndh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 2.940 | 6.330 | 2.940 |
| Rmerge | 0.071 | 0.033 | 0.406 |
| Rmeas | 0.081 | 0.039 | 0.469 |
| Rpim | 0.039 | 0.020 | 0.230 |
| Total number of observations | 50676 | ||
| Number of reflections | 12827 | 1395 | 1206 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.4 | 98 | 96.9 |
| Redundancy | 4 | 3.6 | 3.9 |
| CC(1/2) | 0.997 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100 mM MES, 20% w/v polyethylene glycol 3350 |
