6CVP
Human Aprataxin (Aptx) R199H bound to RNA-DNA, AMP and Zn product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.507, 121.105, 122.314 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.624 - 1.999 |
R-factor | 0.1668 |
Rwork | 0.164 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ndf |
RMSD bond length | 0.011 |
RMSD bond angle | 0.983 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.049 | 0.021 | 0.475 |
Total number of observations | 131390 | ||
Number of reflections | 39638 | 4212 | 3360 |
<I/σ(I)> | 10.5 | ||
Completeness [%] | 97.2 | 96.6 | 83.9 |
Redundancy | 3.3 | 3.5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 100 mM MES, 20% w/v polyethylene glycol 3350 |