6CT9
Structure of the human cGAS-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-11-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 99.782, 99.782, 236.769 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.975 - 2.260 |
R-factor | 0.2021 |
Rwork | 0.199 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4km5 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.210 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rpim | 0.045 | 0.796 |
Number of reflections | 33460 | |
<I/σ(I)> | 10.9 | 1.1 |
Completeness [%] | 99.6 | 96.4 |
Redundancy | 13.1 | 12.1 |
CC(1/2) | 0.998 | 0.317 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES-NaOH pH 7.0, 1.4 M sodium citrate |