6CT9
Structure of the human cGAS-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-11-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 99.782, 99.782, 236.769 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.975 - 2.260 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4km5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.720 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.210 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.045 | 0.796 |
| Number of reflections | 33460 | |
| <I/σ(I)> | 10.9 | 1.1 |
| Completeness [%] | 99.6 | 96.4 |
| Redundancy | 13.1 | 12.1 |
| CC(1/2) | 0.998 | 0.317 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES-NaOH pH 7.0, 1.4 M sodium citrate |
