6CRN

Structure of the USP15 deubiquitinase domain in complex with a high-affinity first-generation Ubv

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 1
Unit cell lengths	41.770, 98.942, 122.043
Unit cell angles	66.49, 88.31, 78.10

Refinement procedure

Resolution	111.720 - 2.500
R-factor	0.1904
Rwork	0.188
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 2Y6E and a SwissModeller model of the 15.13 Ubvc
RMSD bond length	0.008
RMSD bond angle	1.253
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.720	2.140
High resolution limit [Å]	2.110	2.110
Rmerge	0.080	0.815
Number of reflections	92086	612
<I/σ(I)>	7.6	0.8
Completeness [%]	91.2	63.5
Redundancy	2.1	1.9
CC(1/2)	0.993	0.419

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	24% PDG3350, 200 mM Potassium Citrate tribasic, 100 mM MES 6.5, cryoprotected in 21% PEG3350, 100 mM Potassium Citrate tribasic, 100 mM MES 6.5, 25% glycerol