6CPM
Structure of the USP15 deubiquitinase domain in complex with a third-generation inhibitory Ubv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-06-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.552, 115.080, 95.701 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 38.205 - 2.011 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | usp15 and Ubv model for the USP15/AM5 structure |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.067 | 0.507 |
| Number of reflections | 60994 | 6036 |
| <I/σ(I)> | 23.4 | 2.96 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 3.7 | 3.5 |
| CC(1/2) | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 16% PEG3350, 100 mM MES, 200 mM CaCl2, cryoprotected with the identical buffer plus 25% glycerol |
