6CME
Structure of wild-type ISL2-LID in complex with LHX4-LIM1+2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-11-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.488, 88.843, 50.303 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.920 |
| R-factor | 0.20313 |
| Rwork | 0.201 |
| R-free | 0.24343 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 (1.99.2) |
| Data scaling software | Aimless (0.2.14) |
| Phasing software | MOLREP (11.2.05) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.550 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.125 | 0.770 |
| Rmeas | 0.140 | 0.915 |
| Rpim | 0.063 | 0.481 |
| Number of reflections | 29762 | 1441 |
| <I/σ(I)> | 10.2 | 1.3 |
| Completeness [%] | 99.8 | 97.6 |
| Redundancy | 4.6 | 3.2 |
| CC(1/2) | 1.000 | 0.393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 110 mM BisTris propane and 3 M ammonium acetate |
