6CME
Structure of wild-type ISL2-LID in complex with LHX4-LIM1+2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-11-25 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.488, 88.843, 50.303 |
Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.920 |
R-factor | 0.20313 |
Rwork | 0.201 |
R-free | 0.24343 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mmk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-2000 (1.99.2) |
Data scaling software | Aimless (0.2.14) |
Phasing software | MOLREP (11.2.05) |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.550 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.125 | 0.770 |
Rmeas | 0.140 | 0.915 |
Rpim | 0.063 | 0.481 |
Number of reflections | 29762 | 1441 |
<I/σ(I)> | 10.2 | 1.3 |
Completeness [%] | 99.8 | 97.6 |
Redundancy | 4.6 | 3.2 |
CC(1/2) | 1.000 | 0.393 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 110 mM BisTris propane and 3 M ammonium acetate |