6CFN

Crystal Structure of the DNA-free Glucocorticoid Receptor DNA Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	65.803, 65.432, 72.659
Unit cell angles	71.27, 84.96, 68.44

Refinement procedure

Resolution	36.042 - 2.500
R-factor	0.1973
Rwork	0.195
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hn5
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.590
High resolution limit [Å]	2.500	2.500
Rmeas	0.180	0.650
Rpim	0.100	0.460
Number of reflections	33141	2095
<I/σ(I)>	14.5	1.3
Completeness [%]	89.5	56.9
Redundancy	2.4	1.4
CC(1/2)		0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	25% PEG3350, 50 mM Tris pH8.5, and 200 mM di-sodium tartrate