6CFJ

Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	209.780, 449.830, 622.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	152.542 - 2.800
R-factor	0.217
Rwork	0.214
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.542	152.542	2.870
High resolution limit [Å]	2.800	12.520	2.800
Rmerge	0.137	0.017	1.135
Rmeas	0.166	0.020	1.381
Total number of observations	3998432
Number of reflections	1341527	13850	102019
<I/σ(I)>	7.91	35.72	1.07
Completeness [%]	94.0	81.9	97.1
Redundancy	2.981	3.126	2.954
CC(1/2)	0.993	0.999	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME