6CFJ
Crystal structure of the Thermus thermophilus 70S ribosome in complex with histidyl-CAM and bound to mRNA and A-, P-, and E-site tRNAs at 2.8A resolution
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, EB (1A, 2A) | 23S Ribosomal RNA | polymer | 2915 | 948035.6 | 2 | GenBank (37223181) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 2 | B, FB (1B, 2B) | 5S Ribosomal RNA | polymer | 121 | 39188.4 | 2 | GenBank (37223181) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 3 | C, GB (1D, 2D) | 50S ribosomal protein L2 | polymer | 276 | 30532.6 | 2 | UniProt (Q72I07) Pfam (PF00181) Pfam (PF03947) UniProt (by SIFTS) (P60405) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 4 | D, HB (1E, 2E) | 50S ribosomal protein L3 | polymer | 206 | 22450.2 | 2 | UniProt (P52860) Pfam (PF00297) UniProt (by SIFTS) (Q5SHN8) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 5 | E, IB (1F, 2F) | 50S ribosomal protein L4 | polymer | 210 | 23271.9 | 2 | UniProt (Q5SHN9) Pfam (PF00573) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | L1e |
| 6 | F, JB (1G, 2G) | 50S ribosomal protein L5 | polymer | 182 | 21061.6 | 2 | UniProt (P41201) Pfam (PF00281) Pfam (PF00673) UniProt (by SIFTS) (Q5SHQ0) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | TL4,TthL5 |
| 7 | G, KB (1H, 2H) | 50S ribosomal protein L6 | polymer | 180 | 19568.9 | 2 | UniProt (P24316) Pfam (PF00347) UniProt (by SIFTS) (P0DOY8) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 8 | H, LB (1I, 2I) | 50S ribosomal protein L9 | polymer | 148 | 16422.2 | 2 | UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 9 | I, MB (1N, 2N) | 50S ribosomal protein L13 | polymer | 140 | 15927.9 | 2 | UniProt (Q72IN1) Pfam (PF00572) UniProt (by SIFTS) (P60488) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 10 | J, NB (1O, 2O) | 50S ribosomal protein L14 | polymer | 122 | 13323.6 | 2 | UniProt (Q5SHP8) Pfam (PF00238) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 11 | K, OB (1P, 2P) | 50S ribosomal protein L15 | polymer | 150 | 16319.1 | 2 | UniProt (P74910) Pfam (PF00828) UniProt (by SIFTS) (Q5SHQ7) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 12 | L, PB (1Q, 2Q) | 50S ribosomal protein L16 | polymer | 141 | 15993.9 | 2 | UniProt (P60489) Pfam (PF00252) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 13 | M, QB (1R, 2R) | 50S ribosomal protein L17 | polymer | 118 | 13750.1 | 2 | UniProt (Q72I33) Pfam (PF01196) UniProt (by SIFTS) (Q9Z9H5) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 14 | N, RB (1S, 2S) | 50S ribosomal protein L18 | polymer | 112 | 12639.8 | 2 | UniProt (P80320) Pfam (PF00861) UniProt (by SIFTS) (Q5SHQ4) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | TL24 |
| 15 | O, SB (1T, 2T) | 50S ribosomal protein L19 | polymer | 146 | 17188.8 | 2 | UniProt (P60490) Pfam (PF01245) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 16 | P, TB (1U, 2U) | 50S ribosomal protein L20 | polymer | 118 | 13779.3 | 2 | UniProt (Q72L76) Pfam (PF00453) UniProt (by SIFTS) (P60491) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 17 | Q, UB (1V, 2V) | 50S ribosomal protein L21 | polymer | 101 | 11069.2 | 2 | UniProt (Q72HR2) Pfam (PF00829) UniProt (by SIFTS) (P60492) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 18 | R, VB (1W, 2W) | 50S ribosomal protein L22 | polymer | 113 | 12808.1 | 2 | UniProt (P48286) Pfam (PF00237) UniProt (by SIFTS) (Q5SHP3) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 19 | S, WB (1X, 2X) | 50S ribosomal protein L23 | polymer | 96 | 10759.8 | 2 | UniProt (Q9RA57) Pfam (PF00276) UniProt (by SIFTS) (Q5SHP0) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | L*23 |
| 20 | T, XB (1Y, 2Y) | 50S ribosomal protein L24 | polymer | 110 | 12085.6 | 2 | UniProt (Q56435) Pfam (PF00467) Pfam (PF17136) UniProt (by SIFTS) (Q5SHP9) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 21 | U, YB (1Z, 2Z) | 50S ribosomal protein L25 | polymer | 206 | 23238.6 | 2 | UniProt (Q72IA7) Pfam (PF01386) Pfam (PF14693) UniProt (by SIFTS) (Q5SHZ1) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | General stress protein CTC |
| 22 | V, ZB (10, 20) | 50S ribosomal protein L27 | polymer | 85 | 9529.1 | 2 | UniProt (P60493) Pfam (PF01016) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 23 | AC, W (11, 21) | 50S ribosomal protein L28 | polymer | 98 | 11004.2 | 2 | UniProt (P60494) Pfam (PF00830) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 24 | BC, X (12, 22) | 50S ribosomal protein L29 | polymer | 72 | 8670.3 | 2 | UniProt (Q72I12) Pfam (PF00831) UniProt (by SIFTS) (Q5SHP6) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 25 | CC, Y (13, 23) | 50S ribosomal protein L30 | polymer | 60 | 6799.1 | 2 | UniProt (P74909) Pfam (PF00327) UniProt (by SIFTS) (Q5SHQ6) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 26 | DC, Z (14, 24) | 50S ribosomal protein L31 | polymer | 71 | 8300.5 | 2 | UniProt (Q72JR0) Pfam (PF01197) UniProt (by SIFTS) (Q5SJE1) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 27 | AA, EC (15, 25) | 50S ribosomal protein L32 | polymer | 60 | 6722.1 | 2 | UniProt (P80339) Pfam (PF01783) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 28 | BA, FC (16, 26) | 50S ribosomal protein L33 | polymer | 54 | 6632.9 | 2 | UniProt (Q72GW3) Pfam (PF00471) UniProt (by SIFTS) (P35871) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 29 | CA, GC (17, 27) | 50S ribosomal protein L34 | polymer | 49 | 6132.4 | 2 | UniProt (P80340) Pfam (PF00468) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 30 | DA, HC (18, 28) | 50S ribosomal protein L35 | polymer | 65 | 7506.2 | 2 | UniProt (P80341) Pfam (PF01632) UniProt (by SIFTS) (Q5SKU1) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 31 | EA, IC (19, 29) | 50S ribosomal protein L36 | polymer | 37 | 4435.4 | 2 | UniProt (P80256) Pfam (PF00444) UniProt (by SIFTS) (Q5SHR2) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | Ribosomal protein B |
| 32 | FA, JC (1a, 2a) | 16S Ribosomal RNA | polymer | 1521 | 493864.6 | 2 | GenBank (155076) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 33 | GA, KC (1b, 2b) | 30S ribosomal protein S2 | polymer | 256 | 29317.7 | 2 | UniProt (P62662) Pfam (PF00318) UniProt (by SIFTS) (P80371) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 34 | HA, LC (1c, 2c) | 30S ribosomal protein S3 | polymer | 239 | 26751.1 | 2 | UniProt (P62663) Pfam (PF07650) Pfam (PF00189) UniProt (by SIFTS) (P80372) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 35 | IA, MC (1d, 2d) | 30S ribosomal protein S4 | polymer | 209 | 24373.4 | 2 | UniProt (P62664) Pfam (PF00163) Pfam (PF01479) UniProt (by SIFTS) (P80373) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 36 | JA, NC (1e, 2e) | 30S ribosomal protein S5 | polymer | 162 | 17583.4 | 2 | UniProt (P27152) Pfam (PF00333) Pfam (PF03719) UniProt (by SIFTS) (Q5SHQ5) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 37 | KA, OC (1f, 2f) | 30S ribosomal protein S6 | polymer | 101 | 11988.8 | 2 | UniProt (P23370) Pfam (PF01250) UniProt (by SIFTS) (Q5SLP8) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | TS9 |
| 38 | LA, PC (1g, 2g) | 30S ribosomal protein S7 | polymer | 156 | 18051.0 | 2 | UniProt (P62667) Pfam (PF00177) UniProt (by SIFTS) (P17291) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 39 | MA, QC (1h, 2h) | 30S ribosomal protein S8 | polymer | 138 | 15868.6 | 2 | UniProt (P24319) Pfam (PF00410) UniProt (by SIFTS) (P0DOY9) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 40 | NA, RC (1i, 2i) | 30S ribosomal protein S9 | polymer | 128 | 14410.6 | 2 | UniProt (P80374) Pfam (PF00380) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 41 | OA, SC (1j, 2j) | 30S ribosomal protein S10 | polymer | 105 | 11955.0 | 2 | UniProt (P80375) Pfam (PF00338) UniProt (by SIFTS) (Q5SHN7) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 42 | PA, TC (1k, 2k) | 30S ribosomal protein S11 | polymer | 129 | 13737.9 | 2 | UniProt (P62654) Pfam (PF00411) UniProt (by SIFTS) (P80376) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 43 | QA, UC (1l, 2l) | 30S ribosomal protein S12 | polymer | 132 | 14683.5 | 2 | UniProt (P17293) Pfam (PF00164) UniProt (by SIFTS) (Q5SHN3) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 44 | RA, VC (1m, 2m) | 30S ribosomal protein S13 | polymer | 126 | 14338.9 | 2 | UniProt (P62655) Pfam (PF00416) UniProt (by SIFTS) (P80377) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 45 | SA, WC (1n, 2n) | 30S ribosomal protein S14 type Z | polymer | 61 | 7158.7 | 2 | UniProt (P24320) Pfam (PF00253) UniProt (by SIFTS) (P0DOY6) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 46 | TA, XC (1o, 2o) | 30S ribosomal protein S15 | polymer | 89 | 10578.4 | 2 | UniProt (P80378) Pfam (PF00312) UniProt (by SIFTS) (Q5SJ76) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 47 | UA, YC (1p, 2p) | 30S ribosomal protein S16 | polymer | 88 | 10410.0 | 2 | UniProt (P62238) Pfam (PF00886) UniProt (by SIFTS) (Q5SJH3) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 48 | VA, ZC (1q, 2q) | 30S ribosomal protein S17 | polymer | 105 | 12325.7 | 2 | UniProt (P0DOY7) Pfam (PF00366) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 49 | AD, WA (1r, 2r) | 30S ribosomal protein S18 | polymer | 88 | 10258.3 | 2 | UniProt (P62659) Pfam (PF01084) UniProt (by SIFTS) (Q5SLQ0) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 50 | BD, XA (1s, 2s) | 30S ribosomal protein S19 | polymer | 93 | 10605.5 | 2 | UniProt (P80381) Pfam (PF00203) UniProt (by SIFTS) (Q5SHP2) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 51 | CD, YA (1t, 2t) | 30S ribosomal protein S20 | polymer | 106 | 11736.1 | 2 | UniProt (P80380) Pfam (PF01649) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 52 | DD, ZA (1u, 2u) | 30S ribosomal protein Thx | polymer | 27 | 3350.0 | 2 | UniProt (P62612) Pfam (PF17070) UniProt (by SIFTS) (Q5SIH3) | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) | |
| 53 | AB, ED (1v, 2v) | mRNA | polymer | 24 | 7804.7 | 2 | Enterobacteria phage T4 | ||
| 54 | BB, DB, FD, HD (1w, 1y, 2w, 2y) | A-site and E-site tRNAs | polymer | 76 | 24645.9 | 4 | Escherichia coli | ||
| 55 | CB, GD (1x, 2x) | P-site tRNA | polymer | 77 | 24846.9 | 2 | GenBank (205271127) | Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91 | |
| 56 | AAA, AAB, AAC, AAD, ABA... (1A, 1a, 2A, 2y, 1w...) | MAGNESIUM ION | non-polymer | 24.3 | 2605 | Chemie (MG) | |||
| 57 | QVB, SLC, VQA, ZV (2A, 1A) | POTASSIUM ION | non-polymer | 39.1 | 4 | Chemie (K) | |||
| 58 | TLC, XQA (2A, 1A) | N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]-L-histidinamide | non-polymer | 349.3 | 2 | Chemie (EZG) | |||
| 59 | DPC, DYA, EPC, HYA, IPC... (24, 14, 25, 15, 26...) | ZINC ION | non-polymer | 65.4 | 12 | Chemie (ZN) | |||
| 60 | IHB, NYC (1d, 2d) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 2 | Chemie (SF4) | |||
| 61 | ABD, ACD, ADD, BBD, BCD... (1Y, 2B, 2c, 1Z, 2D...) | water | water | 18.0 | 3437 | Chemie (HOH) |
Sequence modifications
1A, 2A: 2 - 2916 (GenBank: 37223181)
1x, 2x: 1 - 76 (GenBank: 205271127)
| PDB | External Database | Details |
|---|---|---|
| G 1273 | Unk 1294 | conflict |
| PDB | External Database | Details |
|---|---|---|
| 4su 8 | G 3317842 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 112 |
| Total formula weight | 4500875.7 | |
| Non-Polymers* | Number of molecules | 2625 |
| Total formula weight | 65657.8 | |
| All* | Total formula weight | 4566533.5 |
