6CE0
Crystal structure of a HIV-1 clade B tier-3 isolate H078.14 UFO-BG Env trimer in complex with broadly neutralizing Fabs PGT124 and 35O22 at 4.6 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2017-10-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 63 |
| Unit cell lengths | 127.351, 127.351, 316.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.866 - 4.602 |
| R-factor | 0.314 |
| Rwork | 0.313 |
| R-free | 0.34090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r26 5cez |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.678 |
| Data reduction software | HKL-2000 (v706e) |
| Data scaling software | HKL-2000 (v706e) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.680 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Number of reflections | 15619 | 798 |
| <I/σ(I)> | 7.7 | 1.25 |
| Completeness [%] | 96.9 | 98 |
| Redundancy | 5.3 | 5.2 |
| CC(1/2) | 0.760 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277.15 | 0.1 Tris (pH 7.4), 0.2 M lithium sulfate, 6% (w/v) polyethylene glycol 4000 |
