6CE0
Crystal structure of a HIV-1 clade B tier-3 isolate H078.14 UFO-BG Env trimer in complex with broadly neutralizing Fabs PGT124 and 35O22 at 4.6 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-10-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 63 |
Unit cell lengths | 127.351, 127.351, 316.350 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.866 - 4.602 |
R-factor | 0.314 |
Rwork | 0.313 |
R-free | 0.34090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r26 5cez |
RMSD bond length | 0.003 |
RMSD bond angle | 0.678 |
Data reduction software | HKL-2000 (v706e) |
Data scaling software | HKL-2000 (v706e) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 4.680 |
High resolution limit [Å] | 4.600 | 4.600 |
Number of reflections | 15619 | 798 |
<I/σ(I)> | 7.7 | 1.25 |
Completeness [%] | 96.9 | 98 |
Redundancy | 5.3 | 5.2 |
CC(1/2) | 0.760 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277.15 | 0.1 Tris (pH 7.4), 0.2 M lithium sulfate, 6% (w/v) polyethylene glycol 4000 |