6CE0
Crystal structure of a HIV-1 clade B tier-3 isolate H078.14 UFO-BG Env trimer in complex with broadly neutralizing Fabs PGT124 and 35O22 at 4.6 Angstrom
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | B | Envelope glycoprotein gp160 | polymer | 140 | 15702.7 | 1 | UniProt (Q2N0T3) UniProt (Q2N0S5) Pfam (PF00517) In PDB | Human immunodeficiency virus 1 | Env polyprotein,Env polyprotein |
2 | G | Envelope glycoprotein gp160 | polymer | 487 | 54100.2 | 1 | UniProt (A4ZPX1) Pfam (PF00516) In PDB | Human immunodeficiency virus 1 | |
3 | D | 35O22 Heavy chain | polymer | 243 | 26171.5 | 1 | Homo sapiens | ||
4 | E | 35O22 Light chain | polymer | 216 | 23318.8 | 1 | Homo sapiens | ||
5 | H | PGT124 Heavy chain | polymer | 236 | 25460.5 | 1 | Homo sapiens | ||
6 | L | PGT124 Light chain | polymer | 214 | 23056.6 | 1 | Homo sapiens | ||
7 | A, C, J | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
8 | F, K | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
9 | I, N | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
10 | M | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
11 | O | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G07617FP) | |||
12 | G | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 13 | Chemie (NAG) |
Sequence modifications
B: 512 - 547 (UniProt: Q2N0T3)
B: 570 - 664 (UniProt: Q2N0S5)
G: 31 - 506 (UniProt: A4ZPX1)
PDB | External Database | Details |
---|---|---|
Asn 548 | - | linker |
Pro 549 | - | linker |
Asp 563 | - | linker |
Trp 564 | - | linker |
Leu 565 | - | linker |
Pro 566 | - | linker |
Asp 567 | - | linker |
Met 568 | - | linker |
Thr 569 | - | linker |
PDB | External Database | Details |
---|---|---|
Cys 605 | Thr 602 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Cys 501 | Ala 500 | engineered mutation |
Gly 507 | - | expression tag |
Gly 508 | - | expression tag |
Gly 509 | - | expression tag |
Gly 510 | - | expression tag |
Gly 511 | - | expression tag |
Ser 512 | - | expression tag |
Gly 513 | - | expression tag |
Gly 514 | - | expression tag |
Gly 515 | - | expression tag |
Gly 516 | - | expression tag |
Ser 517 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 167810.4 | |
Branched | Number of molecules | 9 |
Total formula weight | 6251.7 | |
Non-Polymers* | Number of molecules | 13 |
Total formula weight | 2875.7 | |
All* | Total formula weight | 176937.8 |