6CE0
Crystal structure of a HIV-1 clade B tier-3 isolate H078.14 UFO-BG Env trimer in complex with broadly neutralizing Fabs PGT124 and 35O22 at 4.6 Angstrom
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Envelope glycoprotein gp160 | polymer | 140 | 15702.7 | 1 | UniProt (Q2N0T3) UniProt (Q2N0S5) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein,Env polyprotein |
| 2 | B (G) | Envelope glycoprotein gp160 | polymer | 487 | 54100.2 | 1 | UniProt (A4ZPX1) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 3 | C (D) | 35O22 Heavy chain | polymer | 243 | 26171.5 | 1 | Homo sapiens | ||
| 4 | D (E) | 35O22 Light chain | polymer | 216 | 23318.8 | 1 | Homo sapiens | ||
| 5 | E (H) | PGT124 Heavy chain | polymer | 236 | 25460.5 | 1 | Homo sapiens | ||
| 6 | F (L) | PGT124 Light chain | polymer | 214 | 23056.6 | 1 | Homo sapiens | ||
| 7 | G, H, K (A, C, J) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 8 | I, L (F, K) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 9 | J, N (I, N) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 2 | In PDB GlyTouCan (G22768VO) | |||
| 10 | M (M) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 1 | In PDB GlyTouCan (G81315DD) | |||
| 11 | O (O) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G07617FP) | |||
| 12 | AA, BA, P, Q, R... (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 13 | Chemie (NAG) |
Sequence modifications
B: 512 - 547 (UniProt: Q2N0T3)
B: 570 - 664 (UniProt: Q2N0S5)
G: 31 - 506 (UniProt: A4ZPX1)
| PDB | External Database | Details |
|---|---|---|
| Asn 548 | - | linker |
| Pro 549 | - | linker |
| Asp 563 | - | linker |
| Trp 564 | - | linker |
| Leu 565 | - | linker |
| Pro 566 | - | linker |
| Asp 567 | - | linker |
| Met 568 | - | linker |
| Thr 569 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Cys 605 | Thr 602 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Cys 501 | Ala 500 | engineered mutation |
| Gly 507 | - | expression tag |
| Gly 508 | - | expression tag |
| Gly 509 | - | expression tag |
| Gly 510 | - | expression tag |
| Gly 511 | - | expression tag |
| Ser 512 | - | expression tag |
| Gly 513 | - | expression tag |
| Gly 514 | - | expression tag |
| Gly 515 | - | expression tag |
| Gly 516 | - | expression tag |
| Ser 517 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 167810.4 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 6251.7 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2875.7 | |
| All* | Total formula weight | 176937.8 |
