6CDS
Human neurofibromin 2/merlin/schwannomin residues 1-339 in complex with PIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-14 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.310, 96.590, 106.450 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 48.300 - 2.620 |
| R-factor | 0.191 |
| Rwork | 0.188 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1isn |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.13.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.920 | 2.840 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rpim | 0.119 | 0.971 |
| Number of reflections | 23131 | 2952 |
| <I/σ(I)> | 5.5 | |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 4.2 | 3.6 |
| CC(1/2) | 0.984 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 100 mM HEPES (pH 7.5), 50 mM MgCl2(H2O)6, 35 % PEG 550 monomethyl ether |
