6CAE

Crystal structure of the Thermus thermophilus 70S ribosome in complex with NOSO-95179 antibiotic and bound to mRNA and A-, P- and E-site tRNAs at 2.6A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	209.170, 448.690, 618.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	154.633 - 2.600
R-factor	0.2139
Rwork	0.212
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.004
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	154.633	154.633	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.137	0.031	1.794
Rmeas	0.158	0.036	2.069
Total number of observations	6968917
Number of reflections	1740501	19838	125966
<I/σ(I)>	8.6	34.61	0.85
Completeness [%]	99.0	95.6	97.3
Redundancy	4.004	3.852	3.95
CC(1/2)	0.994	0.997	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME