6C90

Human Mtr4 helicase in complex with ZCCHC8-CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 61
Unit cell lengths	147.635, 147.635, 101.589
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	73.800 - 2.200
R-factor	0.1918
Rwork	0.190
R-free	0.22140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xgj
RMSD bond length	0.002
RMSD bond angle	0.508
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.800	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.640
Number of reflections	63653	6188
<I/σ(I)>	12.2	1.64
Completeness [%]	99.3	96.99
Redundancy	4.6	3.6
CC(1/2)	1.000	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.6 M sodium-potassium (L)-tartrate, 0.1 M Bis-Tris pH 6.5