6C79
Conformational Changes in a Class A Beta lactamase that Prime it for Catalysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2015-01-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 72.720, 72.720, 98.944 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.360 - 1.100 |
Rwork | 0.144 |
R-free | 0.16420 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.360 | 1.160 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.063 | 0.470 |
Rpim | 0.030 | 0.320 |
Number of reflections | 114156 | |
<I/σ(I)> | 6.3 | 1.5 |
Completeness [%] | 92.7 | 90.4 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6.1 | 293 | 10 mg/ml protein in a solution containing 2.0 M ammonium sulfate and 0.1 M sodium citrate (pH 6.1) |