6C78
Substrate Binding Induces Conformational Changes In A Class A Beta Lactamase That Primes It For Catalysis
Experimental procedure
| Experimental method | LAUE |
| Source type | SPALLATION SOURCE |
| Source details | ORNL Spallation Neutron Source BEAMLINE MANDI |
| Synchrotron site | ORNL Spallation Neutron Source |
| Beamline | MANDI |
| Temperature [K] | 293 |
| Detector technology | CMOS |
| Collection date | 2016-01-03 |
| Detector | Hamamatsu C10158DK |
| Wavelength(s) | 2-4 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 73.400, 73.400, 99.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.760 - 1.750 |
| Data reduction software | Mantid |
| Data scaling software | LaueView |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.760 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.153 | 0.229 |
| Number of reflections | 27135 | |
| <I/σ(I)> | 12.2 | 3.3 |
| Completeness [%] | 85.5 | 65.4 |
| Redundancy | 4.89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.1 | 293 | 300 MICROLITERS OF A 10 MG/ML PROTEIN CONCENTRATION IN A SOLUTION CONTAINING 2.0 M AMMONIUM SULFATE AND 0.1 M PREPARED IN D2O, PH 6.1, BATCH MODE, TEMPERATURE 293K |
