6C6X
Crystal structure of Middle-East Respiratory Syndrome (MERS) coronavirus neutralizing antibody JC57-14 isolated from a vaccinated rhesus macaque.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-24 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.698, 129.804, 84.003 |
| Unit cell angles | 90.00, 110.01, 90.00 |
Refinement procedure
| Resolution | 41.280 - 1.990 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.120 | |
| Number of reflections | 103552 | 7096 |
| <I/σ(I)> | 7.71 | |
| Completeness [%] | 90.8 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.09 CHES pH 9.5, 18% PEG 8000 |
