6C5L
Conformation of methylated GGQ in the Peptidyl Transferase Center during translation termination (T. thermophilus)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.023, 452.527, 623.443 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.981 - 3.200 |
R-factor | 0.2241 |
Rwork | 0.223 |
R-free | 0.24740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v5j |
RMSD bond length | 0.004 |
RMSD bond angle | 1.091 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.250 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmeas | 0.439 | 3.111 |
Number of reflections | 970373 | 43805 |
<I/σ(I)> | 10.29 | 1.04 |
Completeness [%] | 100.0 | 100 |
Redundancy | 41.9 | 29.8 |
CC(1/2) | 0.999 | 0.479 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 5 mM HEPES, pH 7.5, 10 mM magnesium acetate, 50 mM potassium chloride, 10 mM ammonium chloride, 6 mM BME |