6C1C
FGFR1 kinase complex with inhibitor SN37116
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 213.293, 52.035, 66.045 |
| Unit cell angles | 90.00, 107.08, 90.00 |
Refinement procedure
| Resolution | 19.510 - 2.150 |
| R-factor | 0.228 |
| Rwork | 0.226 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wun |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.510 | 2.220 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.087 | |
| Rpim | 0.034 | 0.494 |
| Number of reflections | 37994 | 3287 |
| <I/σ(I)> | 19.8 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.5 |
| CC(1/2) | 0.999 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% MPEG 5000, 0.1 M sodium cacodylate pH 7.5, 0.2 M ammonium sulfate |
