6C16

Ubiquitin variant (UbV.Fbl10.1) bound to a human Skp1-Fbl11 fragment complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2017-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	38.241, 119.582, 63.707
Unit cell angles	90.00, 98.45, 90.00

Refinement procedure

Resolution	19.818 - 3.270
R-factor	0.2632
Rwork	0.260
R-free	0.31720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.820	3.530
High resolution limit [Å]	3.270	3.270
Number of reflections	8718	1811
<I/σ(I)>	5.2	1.1
Completeness [%]	98.9	99.3
Redundancy	3.6	3.5
CC(1/2)	0.986	0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	0.1 M malic acid pH 4.5, 0.15 M sodium chloride, 27% (w/v) PEG3350