6BYN
Crystal structure of WDR5-Mb(S4) monobody complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2013-06-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.078, 48.266, 99.825 |
Unit cell angles | 90.00, 107.74, 90.00 |
Refinement procedure
Resolution | 47.580 - 2.690 |
R-factor | 0.21718 |
Rwork | 0.216 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2h9n 3uyo |
RMSD bond length | 0.002 |
RMSD bond angle | 0.686 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.540 | 2.790 |
High resolution limit [Å] | 2.690 | 2.690 |
Rpim | 0.049 | 0.203 |
Number of reflections | 10148 | 953 |
<I/σ(I)> | 20.9 | 4 |
Completeness [%] | 99.7 | 97.44 |
Redundancy | 6.5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 28% PEG3350, 0.1 M BisTris pH 5.5 |