6BYN
Crystal structure of WDR5-Mb(S4) monobody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2013-06-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.078, 48.266, 99.825 |
| Unit cell angles | 90.00, 107.74, 90.00 |
Refinement procedure
| Resolution | 47.580 - 2.690 |
| R-factor | 0.21718 |
| Rwork | 0.216 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h9n 3uyo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.686 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.540 | 2.790 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rpim | 0.049 | 0.203 |
| Number of reflections | 10148 | 953 |
| <I/σ(I)> | 20.9 | 4 |
| Completeness [%] | 99.7 | 97.44 |
| Redundancy | 6.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 28% PEG3350, 0.1 M BisTris pH 5.5 |
