6BYH

Ubiquitin Variant (UbV.Fl11.1) bound to a human Skp1-Fbl11 fragment complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93.15
Detector technology	CCD
Collection date	2016-09-30
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	60.560, 118.188, 146.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.090 - 2.610
R-factor	0.238
Rwork	0.235
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq 5jh5 5ibk
RMSD bond length	0.007
RMSD bond angle	0.859
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.610	2.610
Number of reflections	32211
<I/σ(I)>	24.7	1.7
Completeness [%]	98.5	85.4
Redundancy	6.8	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M PCTP buffer pH6, 25% (w/v) PEG 1500