6BWY

DNA substrate selection by APOBEC3G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.075
Spacegroup name	P 43
Unit cell lengths	79.072, 79.072, 266.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.190 - 2.900
R-factor	0.23665
Rwork	0.234
R-free	0.28692
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ir2 1qzh
RMSD bond length	0.019
RMSD bond angle	1.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.190	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.105	0.856
Rpim	0.064	0.539
Number of reflections	35772	3545
<I/σ(I)>	5.1	1.1
Completeness [%]	97.5
Redundancy	3.3	3.3
CC(1/2)	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	100 mM HEPES pH7, 200 mM LiCl, and 20% (w/v) Polyethylene glycol (PEG) 6000