6BWJ
Crystal structure of the TRPV2 ion channel in complex with RTx
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 90.054, 121.226, 185.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.997 - 3.100 |
R-factor | 0.2488 |
Rwork | 0.247 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5an8 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 185.560 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Rpim | 0.160 | 1.670 |
Number of reflections | 37513 | |
<I/σ(I)> | 8.6 | |
Completeness [%] | 100.0 | |
Redundancy | 13.7 | |
CC(1/2) | 0.990 | 0.330 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | PEG 400, Calcium chloride |