6BOX
Structure of the S. pombe Clr4 catalytic domain bound to SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.625, 71.255, 87.239 |
Unit cell angles | 90.00, 120.37, 90.00 |
Refinement procedure
Resolution | 54.192 - 2.412 |
R-factor | 0.2333 |
Rwork | 0.231 |
R-free | 0.27620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mvh |
RMSD bond length | 0.002 |
RMSD bond angle | 0.532 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.205 | 2.509 |
High resolution limit [Å] | 2.410 | 2.412 |
Rpim | 0.069 | 0.703 |
Number of reflections | 24906 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 96.7 | |
Redundancy | 4.4 | |
CC(1/2) | 0.991 | 0.552 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 200 mM calcium acetate, 100 mM Imidazole, pH 7.5, 15% PEG 8,000 |