6BNK
Crystal structure of TCR-MHC-like molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 79.970, 150.570, 101.200 |
Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
Resolution | 44.450 - 3.200 |
R-factor | 0.2017 |
Rwork | 0.201 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3quz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.450 | 3.330 |
High resolution limit [Å] | 3.200 | 3.200 |
Rpim | 0.102 | 0.300 |
Number of reflections | 39296 | 4451 |
<I/σ(I)> | 4.8 | |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18-20% PEG3350 8% Tacsimate pH 5.0 0.5% dioxane |