6BNK
Crystal structure of TCR-MHC-like molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.970, 150.570, 101.200 |
| Unit cell angles | 90.00, 95.23, 90.00 |
Refinement procedure
| Resolution | 44.450 - 3.200 |
| R-factor | 0.2017 |
| Rwork | 0.201 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3quz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.450 | 3.330 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.102 | 0.300 |
| Number of reflections | 39296 | 4451 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 18-20% PEG3350 8% Tacsimate pH 5.0 0.5% dioxane |
