6BLW
Zinc finger Domain of WT1(+KTS form) with M342R Mutation and 17+1mer Oligonucleotide with Triplet GGT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-18 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.552, 95.572, 83.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.691 - 1.835 |
| R-factor | 0.2233 |
| Rwork | 0.222 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.523 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 1.920 |
| High resolution limit [Å] | 1.835 | 1.835 |
| Rmerge | 0.132 | 0.937 |
| Rpim | 0.022 | 0.210 |
| Number of reflections | 21595 | 1764 |
| <I/σ(I)> | 25.7 | 2.4 |
| Completeness [%] | 96.5 | 80.5 |
| Redundancy | 32.7 | 14 |
| CC(1/2) | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 289 | 30% PEG 300 0.1M CHES:NaOH |
