6BFQ

The mechanism of GM-CSF inhibition by human GM-CSF auto-antibodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	P 1
Unit cell lengths	95.437, 100.535, 101.866
Unit cell angles	91.17, 117.96, 108.65

Refinement procedure

Resolution	50.010 - 2.600
R-factor	0.232
Rwork	0.230
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jix
RMSD bond length	0.014
RMSD bond angle	1.675
Data reduction software	XDS
Data scaling software	Aimless (0.5.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	46.630	2.640
High resolution limit [Å]	2.600	14.220	2.600
Rmerge	0.246	0.097
Rmeas	0.281	0.110
Rpim	0.134	0.051	0.828
Number of reflections	94668	553	4471
<I/σ(I)>	39
Completeness [%]	98.7	95.7	93.4
Redundancy	4.1	4.4	2.7
CC(1/2)	0.967	0.990	0.344

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283.15	0.16 M Calcium acetate, 14.4% PEG8000, 0.08 M Sodium Cacodylate pH 6.5 and 20% glycerol