6BCM
Structure of a Self-inhibited N475A variant of the Venezuelan Equine Encephalitis Virus (VEEV) nsP2 cysteine protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-25 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.527, 45.528, 47.765 |
| Unit cell angles | 78.28, 65.31, 88.81 |
Refinement procedure
| Resolution | 44.460 - 2.100 |
| R-factor | 0.19797 |
| Rwork | 0.195 |
| R-free | 0.24305 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hwk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.427 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 2.180 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 18603 | 2445 |
| <I/σ(I)> | 9.73 | 3.01 |
| Completeness [%] | 97.4 | 97 |
| Redundancy | 3.29 | 2.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M Bicine pH 8.5, 20% PEG 6000, 10% glycerol |
