6BCM
Structure of a Self-inhibited N475A variant of the Venezuelan Equine Encephalitis Virus (VEEV) nsP2 cysteine protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER IMUS MICROFOCUS |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-25 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 42.527, 45.528, 47.765 |
Unit cell angles | 78.28, 65.31, 88.81 |
Refinement procedure
Resolution | 44.460 - 2.100 |
R-factor | 0.19797 |
Rwork | 0.195 |
R-free | 0.24305 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hwk |
RMSD bond length | 0.012 |
RMSD bond angle | 1.427 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.460 | 2.180 |
High resolution limit [Å] | 2.080 | 2.080 |
Number of reflections | 18603 | 2445 |
<I/σ(I)> | 9.73 | 3.01 |
Completeness [%] | 97.4 | 97 |
Redundancy | 3.29 | 2.56 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.1 M Bicine pH 8.5, 20% PEG 6000, 10% glycerol |