6BC0
A Complex between PH Domain of p190RhoGEF and Activated RhoA Bound to a GTP Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 73.160, 73.160, 266.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.610 - 2.198 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.28070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.344 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.610 | 2.277 |
| High resolution limit [Å] | 2.198 | 2.198 |
| Number of reflections | 22370 | 2178 |
| <I/σ(I)> | 24.3 | |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 11.2 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-24% PEG 3350, 100mM TrisCl, 200mM NaCl |
