6BBH
The CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 247.459, 247.459, 210.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.986 - 6.100 |
| R-factor | 0.3165 |
| Rwork | 0.314 |
| R-free | 0.34050 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.200 |
| High resolution limit [Å] | 6.100 | 6.100 |
| Rpim | 0.013 | |
| Number of reflections | 15295 | 763 |
| <I/σ(I)> | 69 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.9 | 23.9 |
| CC(1/2) | 0.376 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 28% PEG 400 150 mM NaCl 100 mM HEPES-NaOH pH 7.5 |
