6B9X

Crystal structure of Ragulator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.12
Spacegroup name	P 61
Unit cell lengths	168.705, 168.705, 52.325
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	146.100 - 1.420
R-factor	0.21196
Rwork	0.212
R-free	0.23703
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vet 3ms6
RMSD bond length	0.008
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	146.103
High resolution limit [Å]	1.425
Number of reflections	271960
<I/σ(I)>	12.12
Completeness [%]	93.6
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M CHES pH 9.0, 40% PEG 600