6B9D
Human ATL1 mutant - R77A bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.058, 68.429, 75.883 |
| Unit cell angles | 117.00, 89.81, 99.04 |
Refinement procedure
| Resolution | 25.180 - 1.950 |
| R-factor | 0.2053 |
| Rwork | 0.204 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q5e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.360 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.079 |
| Rmeas | 0.103 |
| Number of reflections | 63315 |
| <I/σ(I)> | 7.1 |
| Completeness [%] | 96.4 |
| Redundancy | 2.4 |
| CC(1/2) | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M sodium malonate pH 6, 20% PEG3350, 1 mM GDP, 2 mM MgCl2 |
