6B4K

Crystal structure of human DDX19B(AMPPNP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	83.233, 45.364, 127.391
Unit cell angles	90.00, 97.03, 90.00

Refinement procedure

Resolution	42.144 - 2.200
R-factor	0.2086
Rwork	0.207
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ews
RMSD bond length	0.004
RMSD bond angle	0.623
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rpim	0.053	0.979
Number of reflections	48313	4099
<I/σ(I)>	10	1.7
Completeness [%]	99.4	97.9
Redundancy	6.9	6.1
CC(1/2)	0.994	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MIB buffer pH 5.0 (malonate, imidazole, borate), 13 % (w/v) PEG 1500