6B4J
Crystal structure of human Gle1 CTD-Nup42 GBM-DDX19B(AMPPNP) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.940, 73.560, 145.860 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 48.430 - 3.400 |
| R-factor | 0.2656 |
| Rwork | 0.261 |
| R-free | 0.31330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.998 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2006: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rpim | 0.093 | 1.249 |
| Number of reflections | 25636 | 25636 |
| <I/σ(I)> | 6.6 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | |
| CC(1/2) | 0.997 | 0.483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15 % (w/v) PEG 3350, 0.3 M sodium potassium phosphate pH 6.6 |
