6B20
Crystal structure of a complex between G protein beta gamma dimer and an inhibitory Nanobody regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100.15 |
| Detector technology | PIXEL |
| Collection date | 2017-07-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.660, 77.230, 101.660 |
| Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.340 |
| R-factor | 0.20283 |
| Rwork | 0.200 |
| R-free | 0.24906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a0r |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.574 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rpim | 0.220 | 1.770 |
| Number of reflections | 42434 | 3268 |
| <I/σ(I)> | 6.28 | 1.05 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.31 | 4.43 |
| CC(1/2) | 0.991 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 25-30% (w/v) PEG 3350 in 0.1-0.2 M Bis-Tris-HCl, pH 5.5-5.7, and 0.2-0.3 M MgCl2 |
