6B0E

Crystal structure of Pfs25 in complex with the transmission blocking antibody 1260

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-13
Detector	RAYONIX MX300HE
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	53.223, 79.635, 78.065
Unit cell angles	90.00, 90.91, 90.00

Refinement procedure

Resolution	39.818 - 3.300
R-factor	0.233
Rwork	0.230
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z27 5d1x
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	XDS
Data scaling software	XPREP
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.400
High resolution limit [Å]	3.300	3.300
Rpim	0.044	0.362
Number of reflections	9915	835
<I/σ(I)>	14.6	1.5
Completeness [%]	99.8	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	2.0 M ammonium sulfate, 0.1 M Tris pH 8.5