6AZX
Crystal structure of the neutralizing anti-circumsporozoite protein 663 antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.283, 138.085, 206.674 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.931 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a3i |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.584 |
| Data reduction software | XDS |
| Data scaling software | SHELXPREP |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rpim | 0.058 | 0.279 |
| Number of reflections | 67267 | 8643 |
| <I/σ(I)> | 9.56 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 200 mM di-ammonium hydrogen citrate, 20% (w/v) PEG 3350 |
