6AXJ

Crystal structure of the Yaf9 YEATS domain bound to H3K27ac

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	140.318, 140.318, 129.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.079 - 2.379
R-factor	0.1814
Rwork	0.179
R-free	0.22690
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.100
High resolution limit [Å]	2.379
Number of reflections	37536
<I/σ(I)>	11.5
Completeness [%]	98.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277.14	2.0 M Ammonium Sulfate, 0.1 M Bis-Tris