6AXF
Structure of RasGRP2 in complex with Rap1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 138.998, 209.258, 336.765 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.460 - 3.100 |
R-factor | 0.2052 |
Rwork | 0.203 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.460 |
High resolution limit [Å] | 3.100 |
Rmerge | 0.148 |
Number of reflections | 171499 |
<I/σ(I)> | 6.7 |
Completeness [%] | 100.0 |
Redundancy | 4.5 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 17% PEG3350 and 10 mM sodium citrate (pH 5.6) |