6AU6
Crystal structure of GDP-bound human GNAS R201C mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.999907 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.370, 85.580, 125.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.042 - 1.700 |
R-factor | 0.1701 |
Rwork | 0.168 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1azt |
RMSD bond length | 0.005 |
RMSD bond angle | 0.859 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.820 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.082 | 0.342 |
Rpim | 0.035 | 0.149 |
Number of reflections | 44469 | 6189 |
<I/σ(I)> | 12 | 3.8 |
Completeness [%] | 98.4 | 95.7 |
Redundancy | 6.1 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris 8.5, 25% PEG8000, 10 mM TCEP hydrochloride |