6ATT
39S Fab bound to HER2 ecd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-25 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.327, 108.172, 348.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.770 |
| R-factor | 0.25285 |
| Rwork | 0.251 |
| R-free | 0.28855 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3be1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.432 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.870 |
| High resolution limit [Å] | 3.770 | 3.770 |
| Number of reflections | 25241 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.9 | 95 |
| Redundancy | 12.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 295 | PEG8000, ethylene glycol, MES buffer |
