6AT0

Chromodomain HP1 with a p-nitro-L-phenylalanine mutation at position 24 bound to histone H3 peptide containing trimethyl lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	34.417, 76.857, 76.479
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.224 - 1.285
R-factor	0.2418
Rwork	0.241
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kne
RMSD bond length	0.004
RMSD bond angle	0.751
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	11.224
High resolution limit [Å]	1.285
Number of reflections	25429
<I/σ(I)>	12.8
Completeness [%]	96.9
Redundancy	5.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277	0.1M MES, 3.0M (NH4)2SO4