6ASC
Mre11 dimer in complex with Endonuclease inhibitor PFM04
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11583 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.053, 112.682, 81.162 |
Unit cell angles | 90.00, 100.26, 90.00 |
Refinement procedure
Resolution | 46.038 - 2.150 |
R-factor | 0.1984 |
Rwork | 0.197 |
R-free | 0.23180 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.789 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2580: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.150 |
Number of reflections | 43590 |
<I/σ(I)> | 46.71 |
Completeness [%] | 94.2 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | We added 5mM PFM04 (dissolved in DMSO) to 10 mg/ml TmMre11. The mother liquor was 10% PEG3350, 20 mM CaCl2, 0.1 M Mes pH 6.5. This was mixed 1:1 with the protein:inhibitor complex and set up as hanging drop vapor diffusion. For cryoprotection, 20% ethylene glycol was added to the mother liquor. |