6ARP
Structure of a mutant Cetuximab Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.060, 83.070, 211.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.420 - 1.700 |
| R-factor | 0.17284 |
| Rwork | 0.172 |
| R-free | 0.19707 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yy8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.556 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.420 | |
| High resolution limit [Å] | 1.700 | |
| Number of reflections | 124960 | 5833 |
| <I/σ(I)> | 16.2 | 1.8 |
| Completeness [%] | 99.7 | 95 |
| Redundancy | 6.7 | 6.4 |
| CC(1/2) | 0.999 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium citrate, 1.6 M ammonium sulfate, 5% glycerol |
