6APB
Crystal Structure of Non-Neutralizing Infant Antibody ADI-14359 in Complex with Postfusion RSV F Glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-06-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.1809 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.450, 99.040, 323.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.108 - 3.000 |
| R-factor | 0.2233 |
| Rwork | 0.222 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rrt 3qhz 4kmt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.863 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | Aimless (0.5.28) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.110 | 51.110 | 3.080 |
| High resolution limit [Å] | 3.000 | 13.080 | 3.000 |
| Rmerge | 0.449 | 0.095 | 1.662 |
| Rmeas | 0.483 | 0.103 | 1.786 |
| Rpim | 0.177 | 0.040 | 0.650 |
| Total number of observations | 423092 | 5098 | 32769 |
| Number of reflections | 57978 | 810 | 4439 |
| <I/σ(I)> | 5.2 | 10.5 | 1.6 |
| Completeness [%] | 100.0 | 98.6 | 100 |
| Redundancy | 7.3 | 6.3 | 7.4 |
| CC(1/2) | 0.952 | 0.994 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 4.45mg/mL ADI-14359 Fab-postF complex, 13% PEG8000, 0.43M Ammonium Citrate pH 8.5 |
