6AOD
FXIa antibody complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03316 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 136.127, 136.127, 175.956 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 107.900 - 1.800 |
| R-factor | 0.18315 |
| Rwork | 0.182 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kmt |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.059 |
| Data scaling software | XDS (1) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.980 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 2.663 | |
| Number of reflections | 76265 | |
| <I/σ(I)> | 13.99 | |
| Completeness [%] | 99.0 | |
| Redundancy | 8.9 | |
| CC(1/2) | 0.244 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.86 M ammonium sulfate, 8 mM CoCl2, 30 mM K/Na phosphate, pH 6.5 |
