6AO5
Crystal structure of human MST2 in complex with SAV1 SARAH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 223.678, 223.678, 79.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.271 - 2.955 |
| R-factor | 0.2357 |
| Rwork | 0.235 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lgd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.600 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
| Rmerge | 0.124 | 0.051 | 1.715 |
| Rmeas | 0.128 | 0.053 | 1.788 |
| Rpim | 0.029 | 0.012 | 0.483 |
| Total number of observations | 303392 | ||
| Number of reflections | 15976 | 839 | 763 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.9 | 99.3 | 98.5 |
| Redundancy | 19 | 18.4 | 12 |
| CC(1/2) | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05 M NaCl, 0.1 M Hepes, 0.19 mM CYMAL-7, 1 mM TCEP, 40% PEG 400 |
