6AKG
Crystal structure of mouse claudin-3 P134G mutant in complex with C-terminal fragment of Clostridium perfringens enterotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.710, 68.210, 107.880 |
| Unit cell angles | 90.00, 98.70, 90.00 |
Refinement procedure
| Resolution | 49.340 - 4.300 |
| R-factor | 0.2848 |
| Rwork | 0.283 |
| R-free | 0.32780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6akf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.733 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 4.490 |
| High resolution limit [Å] | 4.300 | 4.300 |
| Rmerge | 0.039 | 1.817 |
| Rmeas | 0.042 | 1.965 |
| Rpim | 0.016 | 0.739 |
| Number of reflections | 9133 | 490 |
| <I/σ(I)> | 14.82 | 1.31 |
| Completeness [%] | 88.8 | 53.84 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.999 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293.15 | HEPES, Magnesium nitrate, PEG 2000 MME |
