6AJ4
Crystal structure of the DHR-2 domain of DOCK7 in complex with Cdc42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 196.460, 105.560, 118.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.802 - 3.256 |
R-factor | 0.1876 |
Rwork | 0.184 |
R-free | 0.25880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vhl |
RMSD bond length | 0.011 |
RMSD bond angle | 1.289 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.450 |
High resolution limit [Å] | 3.256 | 3.260 |
Rmeas | 0.120 | 0.960 |
Number of reflections | 73997 | 11796 |
<I/σ(I)> | 11.9 | 1.7 |
Completeness [%] | 99.4 | 97.8 |
Redundancy | 3.9 | |
CC(1/2) | 0.996 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350 and tri-potassium citrate monohydrate |